Pretransfusion testing consists simply of ABO grouping and antibody screening/major crossmatching using the manual Polybrene method without any antiglobulin phase. The manual Polybrene method is performed using reagents prepared in-house and is rapid (about 3 min), inexpensive and easy to perform. Rh(D) typing of patients is unnecessary as the frequency of D in Taiwanese is 99.67% and the occurrence of anti-D in patients (or in haemolytic disease of the newborn) is uncommon.
Great reductions in both cost and labour have resulted from these modifications, and it is suggested that other countries may consider similar modifications.
|Frequency of Rh(D) in Taiwan|