Today most labs are not concerned about detecting complement binding in pretransfusion tests as most clinically significant antibodies can be detected by anti-IgG alone. As a result these labs will accept anticoagulated specimens (plasma) and monospecific anti-IgG for antibody screening. However, if detection of complement binding is deemed to be important, the ways to achieve it are as follows:
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| Ways to Ensure Adequate Complement Levels |