[Click on image ]
[Click on image ]
ABO typing is the most important test in the transfusion laboratory. See fatal hemolytic transfusion reactions , a Medline abstract of a paper on transfusion-associated fatalities reported to the American Bureau of Biologics (1976-1978).
ABO grouping consists of a front and reverse group using known antisera and known red cells respectively. Typing may be done in test tubes, on microtitre plates, or on glass slides/tiles (although the last is not a reliable method and is not done routinely).
Antisera (for the front group)
Red Cells (for the reverse group)
Current pretransfusion protocols allow for confirming ABO compatibility between patient and donor by an immediate spin crossmatch at RT or by an electronic crossmatch, rather than by doing an antiglobulin crossmatch (IAT-XM).
| Determining Blood Groups | 
|