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A Tour of My Lab and the University of Alberta Bad Breath Research Clinic: HPLC Corner |
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Measurement of Salivary Diamines (Cadaverine and Putresceine) |
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Click on Picture for view without arrows |
HPLC involves liquid chromatography at elevated pressures. Salivary diamines are derivatized to allow detection through absorption in the ultraviolet. Derivatization can assist in chromatographic separation. Here we are using a pre-column and a short reversed-phase separating column. HPLC is a highly versatile approach for effective separation of biologically-important substances in secretions such as saliva. This is a resource shared with Dr. Dan Pehowich in our department.
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© 1998 Dr. F.-Michael Eggert, all rights reserved |
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